منابع مشابه
Dual-color photon-counting histogram.
We report on the development of dual-color photon-counting histogram (PCH) analysis. Dual-color PCH is an extension of regular PCH and considers the photon counts received in two detection channels instead of one. Because each detection channel records a different color, dual-color PCH distinguishes fluorescent species not only by differences in their brightness, but also according to their col...
متن کاملThe dual-color photon counting histogram with non-ideal photodetectors.
Dual-color photon counting histogram (PCH) analysis utilizes the photon counts in two detection channels to distinguish species by differences in brightness and color. Here we modify the existing dual-color PCH theory, which assumes ideal detectors, to include the non-ideal nature of the detector. Specifically, we address the effects of deadtime and afterpulsing. Both effects modify the shape o...
متن کاملThe photon counting histogram in fluorescence fluctuation spectroscopy.
Fluorescence correlation spectroscopy (FCS) is generally used to obtain information about the number of fluorescent particles in a small volume and the diffusion coefficient from the autocorrelation function of the fluorescence signal. Here we demonstrate that photon counting histogram (PCH) analysis constitutes a novel tool for extracting quantities from fluorescence fluctuation data, i.e., th...
متن کاملEffect of bin time on the photon counting histogram for one-photon excitation.
We have demonstrated that our photon counting histogram (PCH) model with the correction for one-photon excitation is valid at multiple bin times. The fitted apparent brightness and concentration follow the three-dimensional diffusion model. More importantly, the semi-empirical parameter, F, introduced in the PCH model for one-photon excitation to correct for the non-Gaussian shape of the observ...
متن کاملColor histogram specification by histogram warping
Histogram warping is a novel histogram specification technique for use in color image processing. As a general purpose tool for color correction, our technique constructs a global color mapping function in order to transform the colors of a source image to match a target color distribution to any desired degree of accuracy. To reduce the risk of color distortion, the transformation takes place ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2005
ISSN: 0006-3495
DOI: 10.1529/biophysj.104.048413